《植物生理学报》 2013, 49(9): 923-928

水稻花药对高温胁迫的生理响应

张桂莲^1,2, 张顺堂², 萧浪涛³, 武小金¹, 肖应辉², 陈立云^2,*

^1,#湖南农业大学农学院, 长沙410128; ^2,#深圳市龙岗区农业科技推广中心博士后创新基地, 广东深圳518118; ³湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙410128

通信作者：陈立云；E-mail: chenliyun996@163.com；Tel: 0731-84618757

摘　要：

为了探明水稻花药在高温胁迫下的生理反应及适应机理, 以耐热水稻品系996和热敏感水稻品系4628为材料, 在人工气候室进行高温(9:00~17:00, 37 ℃; 17:00~9:00, 30 ℃)和适温处理(9:00~17:00, 30 ℃; 17:00~9:00, 25 ℃), 研究高温胁迫对水稻花药可溶性糖、可溶性蛋白质、热稳定蛋白、维生素C含量、膜透性及内源激素含量等生理特性的影响。结果表明, 高温使水稻花药中可溶性糖含量、可溶性蛋白质含量和维生素C含量下降, 热稳定蛋白含量上升; 热敏感品系4628花药中丙二醛含量和相对电导率在高温胁迫下增幅大于耐热品系996; 高温胁迫下, 水稻花药中GA3和IAA含量降低, ABA含量上升, 耐热品系996花药中GA3、IAA含量降低幅度小于热敏感品系4628, ABA含量增加幅度小于热敏感品系4628。

关键词：水稻; 花药; 高温胁迫; 生理响应

收稿：2013-05-17   修定：2013-06-17

资助：国家自然科学基金项目(30900874和30971745)、湖南省科技计划博士后项目(2012RS4037)、湖南省自然科学基金项目(11JJ3026)、湖南省教育厅青年基金项目(13B052)和作物学开放基金项目(ZWKF201301)。# 同等贡献单位。

Physiological Responses of Anther to High Temperature Stress in Rices

ZHANG Gui-Lian^1,2, ZHANG Shun-Tang², XIAO Lang-Tao³, WU Xiao-Jin¹, XIAO Ying-Hui², CHEN Li-Yun^2,*

^1,#Collegeof Agronomy, Hunan Agricultural University, Changsha 410128, China; ^2,#Post-Doctoral Innovation Base of Agricultural Science and Technology Promotion Center of Longgang District, Shenzhen, Guangdong 518118, China; ³Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China

Corresponding author: CHEN Li-Yun; E-mail: chenliyun996@163.com; Tel: 0731-84618757

Abstract:

In order to ascertain the physiological reaction and mechanism of adaption to high temperature stress of anther in rice, we treated heat-tolerant line 996 and heat-sensitive line 4628 with high temperature (9:00–17:00, 37 ℃; 17:00–9:00, 30 ℃) treatment and optimal temperature (9:00–17:00, 30 ℃; 17:00–9:00, 25 ℃) treatment in the growth chambers. The soluble sugar content, soluble protein content, heat stable protein con-tent, vitamin C content, membrane permeability and endogenous hormone content of anther in rice were stud-ied. The results showed that soluble sugar content, soluble protein content and vitamin C content of anther de-creased under high temperature stress, but heat stable protein content increased; The increase scope of malondialdehyde (MDA) content and relative conductivity in heat-sensitive line 4628 was greater under high temperature stress than that of in heat-tolerant line 996; Under high temperature stress, GA3 and IAA content of anther decreased, while ABA content of anther increased, the decreasing scope of GA3 and IAA content in heat tolerant line 996 was less than that of heat-sensitive line 4628, the increasing scope of ABA content was less than that of heat-sensitive line 4628.

Key words: rice; anther; high temperature stress; physiological responses